SpectrEcology

Molecular fluorescence spectroscopy is a sensitive and often selective technique. Unlike absorption spectrophotometry, it is not an absolute technique: instruments therefore require calibration before every series of measurements. This may be achieved using a stable reference material, which should absorb and emit at similar wavelengths to the samples of interest. Use of the general purpose fluorescent reference material set type 6BF enables the day to day stability of fluoresence instruments to be verified. The 6BF reference materials are not standards with absolute values, but a set of six relatively stable fluorescent materials in a polymethylmethacrylate matrix with which the stability of the instrument can be monitored. Four blocks exhibit broad band spectra which cover the normally used UV and visible region of the spectrum. Two blocks contain materials suitable for illustrating the selectivity of the technique as well as checking instrument resolution and wavelength calibration.

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